文章摘要

二苯乙烯苷通过PI3K/AKT通路抑制TGFβ诱导的结直肠癌上皮间质转换

作者: 1李品玉, 1刘其礼, 1王敏, 1张霞, 1杨坚
1 肇庆医学高等专科学校病理学与病理生理学教研室,广东 肇庆 526000
通讯: 李品玉 Email: lipingyu24@163.com
DOI: 10.3978/j.issn.2095-6959.10.3978/j.issn.2095-6959.2017.09.006
基金: 肇庆市科技创新指导类项目, 2016040301-22

摘要

目的:探讨二苯乙烯苷(2,3,5,4’-tetrahydroxy stilbene-2-Ο-β-D-glucoside,THSG)对TGFβ诱导的结直肠癌细胞上皮间质转换(epithelial-mesenchymal transition,EMT)的影响及可能的分子机制。方法:通过转化生长因子(transforming growth factor β,TGFβ)诱导HCT116细胞发生EMT,倒置显微镜观察细胞形态变化,Western印迹检测EMT相关分子标志物的表达;划痕实验、细胞迁移实验、倒置显微镜观察不同浓度THSG干预TGFβ刺激对HCT116细胞运动、迁移能力以及形态学的影响,Western印迹检测EMT相关标志物及PI3K/AKT通路的改变。结果:TGFβ刺激HCT116细胞后,细胞由圆形变为长梭形,E-cadherin表达降低,vimentin和N-cadherin表达增加;与对照组相比,THSG可增加E-cadherin和PTEN的表达,降低vimentin和N-cadherin和p-AKT表达,同时抑制细胞的迁移及运动(F=454.723,P<0.001;F=412.161,P<0.001)。结论:THSG可通过PI3K/AKT通路抑制TGFβ诱导的HCT116细胞EMT,并降低其运动迁移能力。
关键词: 二苯乙烯苷 转化生长因子 结直肠癌 PI3K/AKT

2,3,5,4’-tetrahydroxy stilbene-2-Ο-β-D-glucoside inhibits TGFβ-induced epithelial-mesenchymal transition in colorectal cancer via PI3K/AKT pathway

Authors: 1LI Pinyu, 1LIU Qili, 1WANG Min, 1ZHANG Xia, 1YANG Jian
1 Department of Pathology and Pathophysiology, Zhaoqing Medical College, Zhaoqing Guangdong 526000, China

CorrespondingAuthor: LI Pinyu Email: lipingyu24@163.com

DOI: 10.3978/j.issn.2095-6959.10.3978/j.issn.2095-6959.2017.09.006

Abstract

Objective: To investigate the effect of 2,3,5,4’-tetrahydroxy stilbene-2-Ο-β-D-glucoside (THSG) on TGFβ induced epithelial-mesenchymal transition (EMT) in HCT116 cells and further to explore the potential molecular mechanism. Methods: Transforming growth factor β (TGFβ) was used to induce EMT in HCT116 cells, the morphological changes were observed under inverted microscope, and EMT related molecular markers were detected by Western blot. Wound Healing assay, Transwell assay, inverted microscope were performed to detect whether THSG could reverse the motility, migration ability and morphological changes induced by TGFβ, the effect of THSG on EMT-related molecular markers and PI3K/AKT pathway were measured by Western blot. Results: TGFβ treated HCT116 cells transformed from round type to spindle-shaped form, compared with the losing of E-cadherin and increasing of vimentin, N-cadherin. However, combination treatment with THSG neutralized the effect of TGFβ on E-cadherin, vimentin, N-cadherin. Meanwhile, THSG treatment increase the expression of PTEN and suppress p-AKT expression. Moreover, THSG could inhibited TGFβ induced migration and motility ability (F=454.723, P<0.001; F=412.161, P<0.001). Conclusion: THSG can inhibit TGFβ induced EMT in colorectal cancer via PI3K/AKT pathway, and suppress the ability of motility and migration.
Keywords: 2 3 5 4’-tetrahydroxy stilbene-2-Ο-β-D-glucoside transforming growth factor Colorectal cancer PI3K/AKT