文章摘要

CPEB4和VEGF-C在胃癌中的表达及其临床意义

作者: 1田伟峰, 2郎小梅
1 青海省第五人民医院中西结合科,西宁 810007
2 青海省第五人民医院内分泌科,西宁 810007
通讯: 田伟峰 Email: m15297093015@163.com
DOI: 10.3978/j.issn.2095-6959.10.3978/j.issn.2095-6959.2017.09.011

摘要

目的:分析胞质多聚腺苷酸化成分结合蛋白4(cytoplasmic polyadenylation element binding protein 4,CPEB4)和血管内皮生长因子(vascular endothelial growth factor-C,VEGF-C)在胃癌中的表达及其与临床病理因素及预后的关系。方法:Western印迹检测胃癌细胞株及正常胃黏膜细胞株中蛋白的表达,免疫组织化学分析胃癌石蜡标本蛋白的表达,运用统计学方法研究蛋白表达与胃癌临床病理的相关性。运用相关分析分析蛋白表达与临床相关性,Log-rank单因素分析和Cox多因素分析对预后进行分析。结果:Western印迹检测CPEB4和VEGF-C蛋白在GES-1胃黏膜细胞株相对表达量明显低于HGC-27,SGC7901和MGC803胃癌细胞株,差异具有统计学意义(P<0.05)。CPEB4阳性表达率为55.0%,VEGF-C阳性表达率为41.4%;相关分析显示肿瘤大小、肿瘤部位和T分期与CPEB4表达相关,肿瘤大小和N分期与VEGF-C表达密切相关,预后生存Cox多因素分析结果显示分化程度、淋巴结转移N分期、CPEB4表达与VEGF-C表达是胃癌患者的独立预后因素。结论:CPEB4和VEGF-C表达在胃癌的侵袭中起重要作用,其表达为胃癌预后独立因素,可作为胃癌预后指标。
关键词: 胃癌 CPEB4 内皮生长因子C 预后

Value of CPEB4 and VEGF-C expression in patients with gastric cancer

Authors: 1TIAN Weifeng, 2LANG Xiaomei
1 Department of Integrative Medicine, Fifth People’s Hospital of Qinghai Province, Xining 810007
2 Department of Endocrinology, Fifth People’s Hospital of Qinghai Province, Xining 810007, China

CorrespondingAuthor: TIAN Weifeng Email: m15297093015@163.com

DOI: 10.3978/j.issn.2095-6959.10.3978/j.issn.2095-6959.2017.09.011

Abstract

Objective: To investigate the protein expression levels of cytoplasmic polyadenylation element binding protein 4 (CPEB4) and vascular endothelial growth factor-C (VEGF-C) in gastric cancer (GC), and to explore the correlations between these proteins and biological behaviors of GC. Methods: The expression levels of proteins were detected by Western blotting in cell lines and GC tissues. In addition, protein expressions were analyzed by immunohistochemistry in formalin-fixed tissues from GC patients. Data analysis was performed by statistical methods. Results: The protein expression levels of CPEB4 and VEGF-C in GC cell lines were both higher than those in normal gastric mucosa cell line. CPEB4 and VEGF-C protein was mainly localized in the cytoplasm of GC cells. The positive CPEB4 expression ratio in GC tissues was 55.0%, while VEGF-C positive ratio was 41.4%. Size of tumor, tumor location and T stage were identified as the relative factors of CPEB4 expression, while size of tumor and N stage was identified as the relative factors of VEGF-C expression in GC tissues. Cox analysis indicated that degree of differentiation, N stage, CPEB4 expression and VEGF-C expression were independent prognostic indicators for GC patients. Conclusion: CPEB4 and VEGF-C expression were elevated in GC and related to tumor invasion, metastasis and prognosis, and may be promising indicators of prognosis for GC patients.
Keywords: Gastric cancer CPEB4 VEGF-C Prognosis