文章摘要

非小细胞肺癌EGFR突变检测技术的研究进展

作者: 1郭婷, 1王磊, 1李小飞
1 第四军医大学唐都医院胸腔外科,西安 710038
通讯: 李小飞 Email: lxfchest@fmmu.edu.cn
DOI: 10.3978/j.issn.2095-6959.10.3978/j.issn.2095-6959.2017.09.029
基金: 第四军医大学唐都医院基础研究创新基金和苗子人才基金

摘要

针对表皮生长因子受体(epidermal growth factor receptor,EGFR)突变基因的检测是肺癌靶向治疗的重要基石。目前临床中主要针对肺癌组织、脱落细胞以及液态活检标本开展检测工作,此外尿液标本近期也展现出良好的检测效率。针对上述标本,临床中开展多种检测技术,如以特定靶点为目的的技术,包括数字PCR、楔形探针扩增阻滞突变系统等;以筛查为目的的检测技术,包括二代测序(next generation sequencing,NGS)、Sanger测序等,这些技术在特定的标本类型和检测目的中具有独特的优势。一些新兴的技术,如拉曼光谱,也在临床检测中展现出良好的应用前景。
关键词: 肺癌 表皮生长因子受体突变 检测

Research progress in the detection technology of EGFR mutations in non-small cell lung cancer

Authors: 1GUO Ting, 1WANG Lei, 1LI Xiaofei
1 Department of Thoracic Surgery, Tangdu Hospital Affiliated Fourth Military Medical University, Xi’an 710038, China

CorrespondingAuthor: LI Xiaofei Email: lxfchest@fmmu.edu.cn

DOI: 10.3978/j.issn.2095-6959.10.3978/j.issn.2095-6959.2017.09.029

Abstract

The detection of epidermal growth factor receptor (EGFR) mutation gene is an important cornerstone of lung cancer treatment. At present, lung cancer tissue, exfoliative cells and liquid biopsy specimens are used in clinic practice for the detection of EGFR. Even more, the detection of urine specimens also shows potential application prospect. There are many detecting techniques for above specimens, the one of specific targets technology, such as Beaming dPCR, sARMS, and the other one of screening tests technology, such as next generation sequencing (NGS) and Sanger sequencing. The different specific specimen types and the testing purpose for different detecting techniques according to individual advantage. With the development of emerging technology, surface-enhanced Raman scattering shows the great application in clinical practice as the novel detecting technique.
Keywords: lung cancer epidermal growth factor receptor mutation detection