文章摘要

MAPK 信号通路调控子宫内膜癌细胞增殖、侵袭和迁移的影响

作者: 1翁亚菡, 1鲍伟杰, 1梁瑞敏, 1常子涛
1 鹤壁市人民医院病理科,河南 鹤壁 458030
通讯: 翁亚菡 Email: 2123692743@qq.com
DOI: 10.3978/j.issn.2095-6959.2019.03.003

摘要

目的:探讨丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路对人子宫内膜癌细胞HEC-1-B增殖、侵袭和迁移的影响。方法:体外培养人子宫内膜癌细胞HEC-1-B,采用MTT法检测增殖能力,流式细胞仪检测细胞周期,Transwell试验检测细胞侵袭能力,划痕试验检测细胞迁移能力,Western印迹法检测细胞中磷酸化的p38丝裂原活化蛋白激酶(p-p38MAPK)、增殖细胞核抗原(PCNA)、基质金属蛋白酶-2(MMP-2)蛋白表达水平的影响。结果:p38MAPK信号通路抑制剂SB203580对子宫内膜癌细胞HEC -1-B的增殖具有抑制作用,且随着作用浓度的升高抑制作用增强(P<0.05)。与对照组比,施加抑制剂SB203580能够导致G 0/G1期HEC -1-B细胞比例增多,S期和G2/M期细胞比例减少,细胞侵袭抑制率升高,细胞划痕愈合率降低,细胞中p -p38MAPK,PCNA,MMP-2蛋白表达下调,差异具有统计学意义(P<0.05)。且抑制剂SB203580组间比较差异具有统计学意义( P< 0.05)。结论:抑制MAPK信号通路能够抑制人子宫内膜癌HEC-1-B细胞增殖、侵袭和迁移,其作用机制可能与下调细胞中PCNA,MMP-2蛋白表达有关。
关键词: MAPK信号通路;子宫内膜癌细胞;增殖;侵袭;迁移

MAPK signaling pathway regulates proliferation, invasion and migration of endometrial carcinoma cells

Authors: 1WENG Yahan, 1BAO Weijie, 1LIANG Ruimin, 1CHANG Zitao
1 Department of Pathology, People’s Hospital of Hebi, Hebi Henan 458030, China

CorrespondingAuthor: WENG Yahan Email: 2123692743@qq.com

DOI: 10.3978/j.issn.2095-6959.2019.03.003

Abstract

Objective: To investigate the effects of mitogen-activated protein kinase (MAPK) signaling pathway on proliferation, invasion and migration of human endometrial cancer cell line HEC-1-B. Methods: Human endometrial cancer cell line HEC-1-B was cultured in vitro. The proliferation of HEC-1-B cells was detected by MTT assay. The cell cycle of HEC-1-B was detected by flow cytometry. Transwell assay detects the invasive ability of HEC-1-B cells. The scratch test examined the migration ability of HEC-1-B cells. Western blot analysis of p-p38MAPK, PCNA and MMP-2 protein expression levels in cells. Results: p38MAPK signaling pathway inhibitor SB203580 inhibited the proliferation of endometrial cancer cell line HEC-1-B, and the inhibitory effect increased with the increase of concentration (P<0.05). Compared with the control group, the application of the inhibitor SB203580 resulted in an increase in the proportion of G0/G1 phase HEC-1-B cells, a decrease in the proportion of cells in the S phase and the G2/M phase, an increase in the inhibition rate of cell invasion, and a decrease in the rate of cell scratch healing. The expression of p-p38MAPK, PCNA and MMP-2 protein was down-regulated, and the difference was statistically significant (P<0.05). The difference between the inhibitors of SB203580 was statistically significant (P<0.05). Conclusion: Inhibition of MAPK signaling pathway can inhibit the proliferation, invasion and migration of human endometrial carcinoma HEC-1-B cells, and its mechanism may be related to down-regulation of PCNA and MMP-2 protein expression.
Keywords: MAPK signaling pathway; endometrial cancer cells; proliferation; invasion; migration