文章摘要

MiR-377 靶向调控 Egr1 在乙肝肝纤维化病理机制中的作用

作者: 1李贻弘, 1张宏, 1侯波, 1熊首先
1 江汉油田总医院消化科,湖北 潜江 433124
通讯: 熊首先 Email: 616587532@qq.com
DOI: 10.3978/j.issn.2095-6959.2019.03.004

摘要

目的:研究miR-377靶向调控早期生长反应因子(early growth response l,Egr1)在肝纤维化病理机制中的作用。方法:RT-qPCR检测肝纤维化患者血清中miR-377的表达;用miR靶基因数据库进行筛选,选择Egr1为miR-377的潜在靶基因;RT-qPCR和Western印迹法分别检测miR-377 mimics对Egr1的mRNA及蛋白表达的影响;CCK-8检测AngII诱导后HSC细胞和转染miR-377的HSC细胞增殖程度;RT-qPCR检测经AngII和AngII+miR-377刺激后与α-平滑肌动蛋白(α-smooth muscle actin, α-SMA),FSP1和Collagen I的表达量。结果:MiR-377可能与肝纤维化的发生及发展有关;miR-377可以通过结合Egr1的3'-UTR直接抑制Egr1的mRNA翻译及其蛋白表达;miR-377可抑制肝细胞经AngII诱导后的增殖活性;miR-377能抑制经AngII刺激后α-SMA,FSP1和Collagen I的表达,在肝纤维化的进程中发挥抑制作用。结论:MiR-377通过抑制Egr1的表达抑制肝细胞的增殖和病理进程。
关键词: 肝纤维化;miR-377;早期生长反应因子;AngII

Role of miR-377 targeted regulation of EGR1 in the pathological mechanism of liver fibrosis in hepatitis B patient

Authors: 1LI Yihong, 1ZHANG Hong, 1HOU Bo, 1XIONG Shouxian
1 Department of Gastroenterology, Jianghan Oilfield General Hospital, Qianjiang Hubei 433124, China

CorrespondingAuthor: XIONG Shouxian Email: 616587532@qq.com

DOI: 10.3978/j.issn.2095-6959.2019.03.004

Abstract

Objective: To study the role of miR-377 targeted regulation of EGR1 in the pathological mechanism of liver fibrosis. Methods: The expression of miR-377 in serum of patients with liver fibrosis were detected by RT-qPCR. Egr1 was selected as the potential target gene of miR-377 by screening with miR target gene database. The effect of miR-377mimics on the mRNA and protein expression of Egr1 was detected by RT-qPCR and Western blot, respectively. CCK-8 was used to detect the proliferation of HSC cells and HSC cells transfected with miR-377 which were induced by AngII. RT-qPCR was used to detect the expression of α-smooth muscle actin (α-SMA), FSP1 and Collagen I after AngII and AngII + miR-377 stimulated. Results: MiR-377 may be related to the occurrence and development of liver fibrosis. miR-377 can directly inhibit the mRNA translation and protein expression of Egr1 by binding 3'-URT of Egr1. miR-377 could inhibit the proliferation of liver cells induced by AngII. miR-377 could inhibit the expression of α-SMA, FSP1 and Collagen I after AngII stimulated and miR-377 played an inhibitory role in the process of liver fibrosis. Conclusion: MiR-377 inhibits the proliferation and pathological process of liver cells by inhibiting the expression of Egr1.
Keywords: hepatic fibrosis; miR-377; early growth response; AngII