文章摘要

卵巢成人型颗粒细胞瘤腹水的细胞病理观察

作者: 1颜云, 1唐嘉阅, 1刘爽
1 扬州大学附属医院病理科,江苏 扬州 225000
通讯: 颜云 Email: baiyunzhou999@sina.cn
DOI: 10.3978/j.issn.2095-6959.2019.03.011

摘要

目的:探讨卵巢成人型颗粒细胞瘤(adult granulosa cel l tumor,AGCT)腹水的细胞病理特点及诊断要点。方法:分析18例卵巢AGCT腹水液基细胞学诊断、DNA定量及细胞蜡块免疫细胞化学结果,并复习相关文献。结果:AGCT腹水液基细胞学诊断轻度核异质细胞13例,小圆细胞肿瘤5例。液基细胞片中见散在圆形、多角形小细胞,部分成团,核膜有皱褶,核内见明显核沟,染色质疏松泡状或致密,细胞质较少,细胞可裸核状或瓜子状,偶见Call-Exner小体。DNA定量分析均为阴性。免疫细胞化学α-inhibin,S -100,Calretinin,CD99阳性,Ki-67增殖指数为2%~10%。结论:卵巢AGCT腹水联合液基细胞学、DNA定量分析和免疫细胞化学可以得出准确结论,有利于临床诊断和治疗。
关键词: 成人型颗粒细胞瘤;腹水;细胞学;DNA定量分析;免疫细胞化学

Cytopathological observation of ascites in adult granulosa cell tumor

Authors: 1YAN Yun, 1TANG Jiayue, 1LIU Shuang
1 Department of Pathology, Affiliated Hospital of Yangzhou University, Yangzhou Jiangsu 225000, China

CorrespondingAuthor: YAN Yun Email: baiyunzhou999@sina.cn

DOI: 10.3978/j.issn.2095-6959.2019.03.011

Abstract

Objective: To explore the adult granulosa cell tumor ascites cells of the pathological characteristics and diagnostic criteria. Methods: We analyzed 18 cases of ovarian granulosa cell tumor ascites liquid-based cytology in the diagnosis of adult type wax, quantitative analysis of DNA and cell block immunocytochemistry results, and reviewed the relevant literature. Results: AGCT ascites liquid-based cytology in the diagnosis of 13 cases of mild nuclear heterogeneous cells, small round cell tumor in 5 cases. Liquid based cells which are scattered in the round, small polygonal cells, part of the dough, nuclear membrane folds, nucleus in obviously ditch, chromatin loose bubble or density, less cytoplasm, the cell can be naked nucleus shape or melon seeds, occasional Call-Exner corpuscle. DNA quantitative analysis are all negative. Immunocytochemistry, revealed positivity for α-inhibin, S-100, Calretinin and CD99, and Ki-67 index ranges from 2% to 10%. Conclusion: Adult ovary granulosa cell tumor ascites combined liquid-based cytology and DNA double body chemical analysis and immune cell can accurate conclusion, is helpful to clinical diagnosis and treatment.
Keywords: adult granulosa cell tumor; ascites; cytology; DNA quantitative analysis; immunocytochemistry