文章摘要

术前供区拔罐负压吸引促进兔皮瓣成活率的实验研究

作者: 1刘东昕, 1马骏焕, 1夏雪, 1王虎
1 汕头大学医学院第一附属医院骨科,广东 汕头 515041
通讯: 刘东昕 Email: ldx1970@163.com
DOI: 10.3978/j.issn.2095-6959.2022.04.002
基金: 广东省中医药局科研课题面上项目 (20191179)。

摘要

目的:探讨术前供区拔罐负压吸引对兔皮瓣成活率的影响。方法:选取新西兰兔10只,每只兔背部设计4块皮瓣,按照自身对照原则,以背部左侧前方及右侧后方皮瓣(20个)为实验组,背部右侧前方及左侧后方皮瓣(20个)为对照组。实验组手术前7天每天均对皮瓣进行拔罐预处理;对照组未进行任何预处理。测量实验组拔罐前后皮肤温度;比较两组皮瓣成活率,观察HE染色、血管内皮生长因子(vascular endothelial growth factor,VEGF)免疫组织化学染色情况。结果:拔罐负压吸引后皮温显著高于拔罐前(P<0.05);术后第7天,实验组皮瓣成活率高于对照组(P<0.05);HE染色可见实验组较大新生毛细血管形成,VEGF免疫组织化学染色可见实验组皮瓣组织存在大量棕黄色颗粒沉着,两者均优于对照组;实验组皮瓣组织VEGF水平高于对照组(P<0.05)。结论:术前供区拔罐负压吸引能够提高兔移植皮瓣成活率,其作用机制与促进VEGF表达有关。
关键词: 拔罐;负压;皮瓣;成活率

Experimental study on the promoting effect of negative pressure suction of preoperative cupping on the survival rate of rabbit skin flap

Authors: 1LIU Dongxin, 1MA Junhuan, 1XIA Xue, 1WANG Hu
1 Department of Orthopedics, First Affiliated Hospital of Shantou University Medical College, Shantou Guangdong 515041, China

CorrespondingAuthor: LIU Dongxin Email: ldx1970@163.com

DOI: 10.3978/j.issn.2095-6959.2022.04.002

Foundation: This work was supported by the Scienti c Research Project of Guangdong Provincial Bureau of Traditional Chinese Medicine, China (20191179).

Abstract

Objective: To investigate the effect of negative pressure suction of preoperative cupping on the survival rate of rabbit flap. Methods: Ten New Zealand rabbits were selected, and 4 skin flaps were designed on the back of each rabbit. According to the principle of self-control, the left anterior and right posterior back flaps (n=20) were used as the experimental group, and the right anterior and left posterior back flaps (n=20) were used as the control group. In the experimental group, skin flaps were pretreated with cupping every day for seven days before operation. The control group did not undergo any preconditioning. Skin temperature was measured before and after cupping in the experimental group. The survival rate of skin flap was compared between the two groups. HE staining and vascular endothelial growth factor (VEGF) immunohistochemical staining were observed. Results: The skin temperature after negative pressure suction of cupping was significantly higher than that before cupping (P<0.05). Seven days after operation, the flap survival rate of the experimental group was higher than that of the control group (P<0.05). HE staining showed the formation of large new capillaries in the skin flap tissue of the experimental group, and VEGF immunohistochemical staining showed a lot of brown-yellow granules in the skin flap tissue of the experimental group, both of which were superior to those of the control group. The level of VEGF of the experimental group was higher than that of the control group (P<0.05). Conclusion: Preoperative negative pressure suction of cupping in donor area can improve the survival rate of rabbit transplanted skin flap, and the mechanism is related to the promotion of VEGF expression.
Keywords: cupping; negative pressure; skin flap; survival rate