1 山西医科大学第一临床医学院，太原 030001
2 耳鼻咽喉头颈肿瘤山西省重点实验室，太原 030001
3 山西医科大学第一医院检验科，太原 030001
4 山西医科大学第一医院耳鼻咽喉头颈外科，太原 030001
Expression of GPR183 in laryngeal squamous cell carcinoma and its effect on cell proliferation
CorrespondingAuthor: YANG Zehua Email: email@example.com
Foundation: This work was supported by the National Natural Science Foundation of China (81872210).
Objective: To investigate the expression of G protein-coupled receptor 183 in laryngeal squamous cell carcinoma (LSCC) and its influence on cell proliferation. Methods: The level of GPR183 expression in LSCC samples (n=42) and paracancer tissues samples (n=5) were assessed by immunohistochemistry (IHC), and the relationship between GPR183 expression and clinicopathological parameters of patients were statistically evaluated. The expression level of GPR183 in HEK293T cells and laryngeal squamous cell carcinoma FD-LSC-1 and AMC-HN-8 cells were detected by quantitative reverse transcription-quantitative polymerase chain reaction (RT-qPCR). After transfection of GPR183 small interfering RNA (siRNA) into laryngeal squamous cell carcinoma cell lines (FD-LSC-1, AMC-HN-8). qRT-PCR was used to detect transfection effect, CCK8 cell proliferation assay and cell clone formation assay were used to detect cell proliferation, and flow cytometry was used to detect cell-cycle progression. Additionally, in vivo experiments were performed to evaluate the effect of GPR183 on the growth of laryngeal squamous cell carcinoma. Results: The expression levels of GPR183 was significantly upregulated in laryngeal squamous cell carcinoma. The expression of GPR183 in laryngeal squamous cell carcinoma was correlated with clinical T stage (P<0.001) and lymph node metastasis (P=0.002). Downregulating GPR183 expression level in LSCC cell lines can significantly inhibit the proliferation of tumor cells, and induce cell cycle arrest at G1 phase. In vivo experiments showed that knockdown GPR183 delayed the growth of laryngeal squamous cell carcinoma. Conclusion: GPR183 was over-expressed in laryngeal squamous cell carcinoma. Down regulation of GPR183 can inhibit the proliferation of laryngeal squamous cell carcinoma.