文章摘要

长非编码反义RNA FMR1-AS1及其正义RNA FMR1在宫颈癌中的表达

作者: 1黄秋霞, 1欧海玲, 1黄克强, 1刘姗姗
1 广西中医药大学第一附属医院病理科，南宁 530023
通讯: 欧海玲 Email: ohl402@126.com
DOI: 10.3978/j.issn.2095-6959.2022.09.004
基金: 广西壮族自治区卫生和计划生育委员会科研课题(Z2016658)。

摘要

目的：通过整合计算多中心样本研究长非编码反义RNA FMRP翻译调节因子1反义RNA 1 (FMRP translational regulator 1 antisense RNA 1，FMR1-AS1)与相应正义RNA FMR1在宫颈癌中的表达。方法：从癌症基因组图谱(The Cancer Genome Atlas，TCGA)和基因表达综合(Gene Expression Omnibus，GEO)数据库检索并下载宫颈癌相关高通量数据集。箱图和独立样本t检验用于比较宫颈癌组织与非癌宫颈组织中FMR1-AS1、FMR1的表达差异。整合计算标准化均数差(standard mean difference，SMD)用于综合探究FMR1-AS1、FMR1在宫颈癌中的表达水平。使用Pearson相关分析研究FMR1-AS1与FMR1表达的相关性。基因集富集分析(gene set enrichment analysis，GSEA)用于研究相关的信号通路。结果：合并SMD的结果显示FMR1-AS1在宫颈癌组织中高表达(SMD=0.63，95%CI：0.15~1.11)。同时，合并1 018例样本(678例宫颈癌组织，340例正常宫颈组织)的结果表明FMR1在宫颈癌组织中的表达显著高于正常宫颈组织(SMD=0.49，95%CI：0.32~0.67)。Pearson相关分析表明：FMR1-AS1与FMR1在宫颈癌组织中的表达呈显著正相关(Pearson’s r=0.4463，P<0.00001)。GSEA结果表明FMR1-AS1和FMR1可能参与基因表达调控相关通路。结论：FMR1-AS1与FMR1在宫颈癌组织中的表达显著上调且两者呈正相关，两者可能共同发挥促癌作用，值得进一步研究二者潜在的调控机制。
关键词： FMR1-AS1；反义RNA；长非编码RNA；FMR1；宫颈癌

Expression levels of long non-coding antisense RNA FMR1-AS1 and its sense-strand RNA FMR1 in uterine cervical cancer

Authors: 1HUANG Qiuxia, 1OU Hailing, 1HUANG Keqiang, 1LIU Shanshan
1 Department of Pathology, First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China

CorrespondingAuthor: OU Hailing Email: ohl402@126.com

DOI: 10.3978/j.issn.2095-6959.2022.09.004

Foundation: This work was supported by the Scientific Research Project of the Health and Family Planning Commission of Guangxi Zhuang Autonomous Region, China (Z2016658).

Abstract

Objective: To explore the expression level of long non-coding antisense RNA FMRP translational regulator 1 antisense RNA 1 (FMR1-AS1) and its relative sense-strand RNA FMR1 in uterine cervical cancer (UCC) through integrated calculation. Methods: UCC-related high-throughput datasets were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases. Boxplots and Student’s t-test were conducted to compare the differences of FMR1-AS1 and FMR1 expression in UCC and normal cervical tissues. Pooled standard mean difference (SMD) was utilized to investigate the expression level of FMR1-AS1 and FMR1 in UCC. Pearson’s correlation analysis was performed to study the correlation of FMR1-AS1 and FMR1 expression in UCC. Gene set enrichment analysis (GSEA) was used to discuss the relative signaling pathway. Results: The result of pooled study revealed that FMR1-AS1 was overexpressed in UCC (SMD=0.63, 95%CI: 0.15 to 1.11). Meanwhile, the expression of FMR1 in UCC was also higher than that in normal cervical tissues via combination of 1 018 samples (678 cases of UCC and 340 cases of normal cervical tissues; SMD=0.49, 95%CI: 0.32 to 0.67). The result of Pearson’s correlation analysis demonstrated that expression of FMR1-AS1 and FMR1 was positively correlated in UCC. The results of GSEA revealed that FMR1-AS1 and FMR1 may participate the regulation of gene expression pathway. Conclusion: FMR1-AS1 and FMR1 were significantly upregulated in UCC tissues and were positively correlated. FMR1-AS1 and FMR1 may jointly facilitate tumorigenesis in UCC, which deserves further research.

Keywords： FMR1-AS1; antisense RNA; long non-coding RNA; FMR1; uterine cervical cancer

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